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. 2002 Jul 1;16(13):1682–1695. doi: 10.1101/gad.983802

Figure 2.

Figure 2

Configuration of markers flanking loxP sites before and after Cre-mediated recombination as determined by PCR. (A) Oligonucleotide primers (1, 2, 3, 4, and 5) give unique sized products. Oligos 1, 2, 3, and 4 are described elsewhere (Burgess and Kleckner 1999). Oligo 5 is 5′-CATCGTATGCCAAAGTCC-3′ and anneals to the ADE2 gene. Parental markers and two expected types of recombinant marker configurations following equational chromosome segregation after RTG are diagramed (see text for details). (B) Expected size of PCR products for nonprototrophs (parental class) and prototrophic strains (recombinant class). (C) Number of observed PCR product classes among Ura+ prototrophs (top) and Ade+ prototrophs (bottom) analyzed by RTG 8 h after transfer into SPM (7 h after Cre-induction) in wild-type, ime2Δ, and spo11Δ strains.