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. 2002 Jul 1;16(13):1610–1615. doi: 10.1101/gad.229402

Figure 2.

Figure 2

Nuclear localization and interactions of BDL protein. (A–F) Transient expression of 35S∷GFP-BDL in parsley protoplasts. (A–C) GFP fluorescence, (D–F) corresponding bright-field illumination images of (A,D) GFP-BDL, (B,E) GFP with nuclear localization signal, and (C,F) GFP with nuclear export signal. Note strong accumulation of BDL-GFP in patches within the nucleus. (G) Yeast two-hybrid interaction assays. The strength of interaction is shown in activity units of the β-galactosidase reporter (β-Gal). BDL (IAA12) interacts with itself and with the C terminus of MP (ARF5) comprising domains III and IV (ARF5Ct). Negative controls: BDL tested against the empty prey vector (IAA12/−), and GNOM tested against the C terminus of MP (GN/ARF5Ct).