Figure 1.

Schematic representation of the recombinant proteins tested in the two hybrid system. LexA, DNA binding domain of the bacterial repressor LexA. TnT-wt, wild-type human fast skeletal βTnT. TnTΔ203–214, βTnT mutant in which amino acid residues 203–214 have been deleted. TnT-G²⁰³S²⁰⁴, βTnT mutant in which two amino acids, Leu²⁰³ and Trp²⁰⁴ have been changed to Gly and Ser, respectively. TnT-R²⁰⁷, βTnT mutant in which Leu²⁰⁷ has been changed to Arg. TnT_150–258, βTnT fragment containing amino acid residues 150–258. TnI-wt, rabbit fast skeletal TnI. TnI_58–10, TnI fragment from amino acid residues 58–108. TnC, rabbit skeletal TnC. Act, transcriptional activation domain. For details see also Materials and Methods.