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BLM binds selectively to synthetic X-junctions. Band-shift assays were carried out as described in Methods using 0.5 nM substrate representing either 32P-labeled X-junction (lanes a–e), 3′-tailed duplex DNA (lanes f–j), ssDNA (lanes k–o), or blunt-ended dsDNA (lanes p–t), together with the indicated concentrations of BLM.
