Combinatorial effect of TNF-α with FGF-2, dexamethasone or estradiol on prolactin promoter activity.
GH3/hPrl-luc cells were serum starved for 24h and then stimulated for 6h or 24h with (A) FGF-2 (10 ng/ml), (B) dexamethasone (10 nM), or (C) estradiol (0.1 nM) alone, in combination with TNF-α (10 ng/ml), or TNF-α alone. Inset (B): GH3 cells were transfected with the pNFκB-luc reporter construct, serum starved for 24h and treated with TNF-α and/or dexamethasone for 6h. Results are shown as means ± SD of at least three independent experiments; * P < 0.05.