Nlk expression in adult and embryonic tissue. (A) Polyadenylated RNA isolated from adult mouse brain (Br), heart (He), intestine (In), kidney (Ki), liver (Li), and lung (Lu), was probed with the entire Nlk cDNA (Upper), or rat GAPDH (Lower). 28S and 18S ribosomal RNAs were used as markers. (B) Polyadenylated RNA isolated from entire mouse embryos, from days E9.5–E18.5, was electrophoresed, transferred to membranes, and probed with the entire Nlk cDNA (Upper), or rat GAPDH (Lower). 28S and 18S ribosomal RNAs were used as markers.