Figure 1.

eor-1 encodes a PLZF-related BTB/zinc-finger protein. (A) Gene structure of eor-1 based on analysis of cDNA clone yk87e7. Coding sequences are shaded black. (B) Amino acid sequence of EOR-1. Arrowheads indicate the sites of the cs28, cs40, and cs44 lesions. The BTB domain is boxed with a solid line. The nine C₂H₂ zinc-finger domains are underlined, and the cysteines and histidines are in bold. The polyglutamine stretch is underlined twice. Potential nuclear localization sequences are boxed with dashed lines. (C) EOR-1 BTB domain alignment. Conserved regions are boxed. Shaded areas are identical residues. An asterisk indicates a conserved leucine changed to a phenylalanine in eor-1(cs44). The EOR-1 BTB domain is 31% identical and 45%–55% similar to the BTB domains of mammalian PLZF, Drosophila kelch, and Drosophila Tramtrack (Ttk). (D) Alignment of zinc-finger domain regions of EOR-1 and PLZF. EOR-1 and PLZF are 30% identical and 45% similar over this region. The nine C₂H₂ zinc-finger domains are underlined, and the cysteines and histidines are indicated with dots.