Figure 3.

Cyclin D3–Cdk4 does not inhibit the ability of CaMKIV to exclude class II HDAC molecules from the nucleus. 10T1/2 fibroblasts were transfected with plasmids encoding HDAC7-HA alone (A–C), or in combination with either activated CaMKIV (D–F) or activated CaMKIV plus cyclin D3 and cdk4 (G–L). Then, 48 h after transfection, the cells were fixed and stained for HDAC7-HA (B,E,I,J) and cyclin D3 (C,F,K,L). Nuclei were visualized by staining with DAPI (A,D,G,H).