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GRIP-1 and MEF2C colocalize to identical punctate subnuclear domains. 10T1/2 fibroblasts were transfected with plasmids encoding GRIP-1–HA and MEF2C–Flag. Then, 48 h after transfection, the cells were fixed and stained for GRIP-1–HA (B,D) and MEF2C–Flag (C,E). Nuclei were visualized by staining with DAPI (A). D and E are highly contrasted image acquisitions of B and C. The arrows indicate punctate subnuclear domains where GRIP-1 and MEF2C colocalize.
