Table 1.
Cyclin D3–cdk4 blocks the localization of GRIP-1 to punctate nuclear subdomains
| Plasmid transfected
|
No. of cells counted
|
C3H10T1/2 cells showing a GRIP-1-HA nuclear staining
|
|
|---|---|---|---|
| Punctate nuclear
|
Diffuse nuclear
|
||
| GRIP-1-HA + vehicles | Exp 1 = 200 | 35% | 65% |
| Exp 2 = 200 | 25% | 75% | |
| Exp 3 = 100 | 36% | 64% | |
| GRIP-1-HA Cyclin D3, Cdk4 + vehicles | Exp 1 = 200 | 2% | 98% |
| Exp 2 = 200 | 4% | 96% | |
| Exp 3 = 100 | 5% | 95% | |
| GRIP-1-HA MEF2C + vehicles | Exp 1 = NDa | — | — |
| Exp 2 = 200 | 31% | 69% | |
| Exp 3 = 100 | 41% | 59% | |
| GRIP-1-HA Cyclin D3, Cdk4 MEF2C | Exp 1 = NDa | — | — |
| Exp 2 = 200 | 5% | 95% | |
| Exp 3 = 100 | 8% | 92% | |
10T1/2 cells were cotransfected with the indicated plasmids and subsequently fixed and immunostained for the localization of transfected GRIP-1–HA. Cells showing either punctate or diffuse nuclear staining of GRIP-1–HA were tailed in each experiment.