Figure 1.

RAD9, MEC1, and CHK1 inhibit growth of yku70Δ strains at high temperatures. Small aliquots of fivefold dilution series of several yeast strains were transferred to plates and incubated at the temperatures indicated for 2 d before being photographed. The relevant genotypes of the strains are indicated on the left, and the strain numbers shown in parentheses. The mec1Δ strains also carried an sml1Δ mutation. To ensure reproducibility we routinely examined two independent strains with the same genotypes. All strains grouped together were grown on the same plates, except for the yku70Δ dun1Δ strains, which were grown on different plates. The photographs of the yku70Δ dun1Δ strains were superimposed in g,h,i.