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. 2002 Aug 1;16(15):1919–1933. doi: 10.1101/gad.225102

Figure 2.

Figure 2

RAD9, MEC1, and CHK1 cause G2/M arrest of yku70Δ strains at 37°C. A series of yeast strains dividing exponentially at 23°C were placed at 37°C and their growth and cell cycle distribution were monitored. At indicated time points, cells density was determined by hemocytometer (a–d) and cell cycle distribution was determined by staining with DAPI (e–h). The yeast stains used were YKU+ RAD+: DLY640 in a,d,e,h, and DLY641 in b,c,e,f; yku70Δ: DLY1412 in a,e, and DLY1366 in b,c,d,f,g,h; yku70Δ rad24Δ: DLY 1505; yku70Δ dun1Δ: DLY 1553; ddc1Δ: DLY883; yku70Δ ddc1Δ: DLY1220; chk1Δ: DLY1095; yku70Δ chk1Δ: DLY1215; sml1Δ: DLY1248; mec1Δ: DLY1249; yku70Δ mec1Δ rad24Δ: DLY1327; and yku70Δ mec1Δ: DLY1325.