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. 2002 Aug 1;16(15):1919–1933. doi: 10.1101/gad.225102

Figure 4.

Figure 4

MAD2 inhibits the growth of yku70Δ mutants, while BUB2 inhibits growth of cdc13-1 mutants. (a–l) Small aliquots of fivefold dilution series of several yeast strains were transferred to plates and incubated for 2 d (a–f) or 3 d (g–l) before being photographed. The relevant genotypes of the strains are indicated on the left and the strain numbers shown in parentheses. (m,n) A series of yeast strains dividing exponentially at 23°C was placed at 37°C and their growth and cell cycle distribution were monitored. At indicated time points, cell density was determined by hemocytometer and cell cycle distribution was determined by staining with DAPI. The yeast stains used were YKU+ RAD+: DLY640; yku70Δ chk1Δ mad2Δ: DLY1446; yku70Δ chk1Δ: DLY1215; yku70Δ mad2Δ: DLY1445; yku70Δ: DLY1412; and yku70Δ bub2Δ: DLY1440.