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. 2002 Aug 1;16(15):1919–1933. doi: 10.1101/gad.225102

Figure 6.

Figure 6

EXO1 is required for arrest and ssDNA generation in yku70Δ mutants. (a) Small aliquots of fivefold dilution series of several yeast strains were transferred to plates and incubated at 28°C, 36°C, or 37°C for 2 d before being photographed. The relevant genotypes of the strains are indicated on the left, and the strain numbers shown in parentheses. (b) A yku70Δ exo1Δ (DLY1408) yeast strain dividing exponentially at 20°C was placed at 37°C, and its cell cycle distribution was monitored by staining with DAPI (see Figs. 2 and 4n for the behavior of control strains). (c) Detection of ssDNA on the TG strand 600 bases from the telomeric end of the Y‘ sequence. The yeast strains were yku70Δ: DLY1412; yku70Δ exo1Δ: DLY1408; and YKU70+: DLY640. (d) Detection of ssDNA on the AC strand 600 bases from the telomeric end of the Y‘ sequence. The yeast strains are as in c.