Figure 3.

Impaired RNAi in aub and spn-E mutant oocytes. bicoid mRNA levels were measured by RT-PCR and were normalized to corresponding RP49 mRNA levels for each sample. (A) bicoid dsRNA was injected into oocytes after in vitro activation (open bars). Control oocytes were mock-injected (filled bars). RNA was extracted 100 min after injection. The strains used were wild-type, spn-E⁶¹⁶/spn-E⁶¹⁶, and aub^QC42/aub^QC42. The error bars show standard error values. (B) bicoid dsRNA was injected into mature activated oocytes that had been collected from virgin females (open bars). Control oocytes were mock-injected. RNA was extracted 60 min after injection. The strains were wild-type, spn-E⁶¹⁶/spn-^Ehls-Δ125, aub^QC42/aub^HN2, and vasa^PD23/vasa^PD23. The error bars show standard error values. (C) hunchback dsRNA was injected into mature activated oocytes that had been collected from virgin females (open bars). Control oocytes were mock-injected. RNA was extracted 80 min after injection. The strains were wild-type, spn-E⁶¹⁶/spn-E^hls-Δ125, and aub^QC42/aub^HN2. The error bars show standard error values.