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. 2002 Aug 1;16(15):1934–1949. doi: 10.1101/gad.984502

Figure 2.

Figure 2

Formation of the 12-SC and the paired complex. (A) EMSA for detection of the 12-SC was performed using MBP-RAG1, GST-RAG2, and HMG2 proteins in 5 mM Ca++ with a 5′ end-labeled double stranded 12-RSS oligonucleotide substrate. Complexes were resolved on a native 4% polyacrylamide gel. (B) The assay for paired complex formation was performed with labeled 12-RSS in a buffer containing Ca++ in the presence or absence of unlabeled 23-RSS and HMG2 as indicated. Complexes were analyzed on a native 6% (80:1) polyacrylamide gel. The positions of the bands corresponding to the 12-SC as well as the paired complex are indicated at the right.