Figure 5.

AtPP2C provides redundant functions in ABA signaling. (A) AtPP2C, ABI1, and ABI2 possess two uniquely conserved G residues around the DGH (underlined) active site. Cotransfection was performed with HVA1-LUC and an inert plasmid or with four different plasmids expressing the wild-type AtPP2C (wt), the N-terminal deletion dAtPP2C (d), the null mutant AtPP2Cn (n), and the dominant mutant AtPP2Ci (i). LUC activity (B) and PP2C activity (C) were determined from the extracts of maize mesophyll protoplast harvested 3 h after DNA transfection.