Figure 6.
YaeL depletion prevents activation of σE-pathway on OmpC overexpression. Plasmid pKK74 (ompC; solid symbols) and pMPM-T1 (vector; open symbols) were introduced into KK360 (λ[rpoHP3-lacZ] yaeL∷kan/pKK6 [yaeL+]). Cells were grown at 30°C in L − 0.02% arabinose medium. Cells at a mid log phase were harvested, washed five times by repeated centrifugation/suspension with arabinose-free L medium, and inoculated into L-arabinose medium (triangles) or arabinose-free L medium (circles). After further incubation for 2 h at 30°C, isopropyl-β-d-thiogalactopyranoside (final concentration, 1 mM) and cyclic AMP (final concentration, 1 mM) were added to induce the OmpC overexpression. At the indicated time points, samples were withdrawn and assayed for β-galactosidase activity.