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Pathway for the unfolding of the 1TIT domain. (A) X-ray structure, rNC = 45 Å; B–D temperature-induced unfolding pathway; (B) 0.6 ns, rNC = 34 Å, (C) 1.5 ns, rNC = 37 Å; (D) 4.8 ns, rNC = 38 Å. E–H forced unfolding pathway; (E) rNC = 54 Å; (F) rNC = 75 Å; (G) rNC = 126 Å; (H) rNC = 138 Å. Native elements of secondary structure are shown by using the same color code as in Fig. 1; nonnative are in grey.
