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. 2002 Aug 15;16(16):2097–2107. doi: 10.1101/gad.1007602

Figure 2.

Figure 2

Meis1 and Meis2 binding to the 26-bp Pax6 lens element sequence. (A) After blotting λgt11 clones to nitrocellulose and probing with wild-type or mutant radioactive ds-oligonucleotides, phage clones expressing DSEB (a non-specific DNA binding protein; Stuempfle and Floros 1997) bind both wild-type and mutant C probes (left), whereas chicken Meis2-expressing clones specifically bind the wild-type 26-bp probe but not the mutant C probe (right). (B) Mouse Meis1 protein binds the 26-bp element. HA-tagged Meis1 forms a gel-shift complex with the 26-bp probe (lower arrow), that is supershifted (upper arrow) when anti-HA antibody is added.