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Meis constructs used in electroporation are based on the cDNA for Meis1a (kindly provided by Dr. M. Cleary; Jacobs et al. 1999). The En or VP16 domains confer repressor or activator activity, respectively upon the fusion proteins. (MD) Meis domain; (En) Drosophila Engrailed repressor domain; (HD) Meis Homeodomain; (VP16) activation domain of virion protein 16 of herpes simplex virus. (For detailed information on the constructs or their preparation, contact R. Maas.)
