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. 1998 Feb 3;95(3):981–986. doi: 10.1073/pnas.95.3.981

Figure 1.

Figure 1

Limited proteolysis of the purified RecB protein (A) and purification of the RecB1–929 deletion mutant (B). (A) The RecB protein (100 μg) was digested by adding subtilisin (0.09 μg) in 0.2 M potassium phosphate, pH 6.8/5 mM DTT at 37°C. Samples were analyzed on a 10% SDS/PAGE gel. (B) SDS/PAGE analysis of the purified RecB1–929 protein (lane 1), partially digested RecB (lane 2), and the purified 100-kDa fragment from limited proteolysis (lane 3).