Figure 1.

Relationship between BAEC morphology and average cell speeds. BAEC were grown to confluence, wounded with a cell scraper, and allowed to recover overnight. The trajectory taken by individual cells was analyzed in time-lapse recordings. (A) Recovering wounded BAEC monolayers exhibit four distinct cell morphologies: rare isolated cells free to move in any direction; polarized wound-edge cells with broad lamellae; elongated cells aligned perpendicular to the wound; and the cobblestone morphology typical of confluent BAECs. (B) Each BAEC phenotype has a distinct rms cell speed. Error bars are SEMs with 20 < n < 50.