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Far-UV CD spectra of ASTm1–142 and Tm143–284(5OHW269) at various concentrations of TFE. The analyzed fragment is indicated above each panel. Conditions: 54.5 mM MOPS, pH 7.0, 0.5 mM EDTA, 1 mM DTT at 25°C. Solid line, 0% TFE/glycerol; dotted line, 7.5% TFE/glycerol; broken line, 15% TFE/glycerol.
