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Concentration dependence of thermal denaturation of ASTm1–142 and Tm143–284(5OHW269) in the absence and presence of 15% TFE. Ellipticities (222 nm) as a function of temperature (4°C–80°C) measured at three protein concentrations (dimer). Solid line, 2 μM protein; dotted line, 10 μM protein; and broken line, 30 μM protein. Conditions: 54.5 mM MOPS, pH 7.0, 0.5 mM EDTA, 1 mM DTT, ±15% TFE (v/v).
