TABLE 1.

Urea denaturation parameters for the Tm fragments as a function of TFE and glycerol concentration

	[urea]1/2 (M)	m (Kcal mol−1 M−1)
ASTm1–142	2.88	1.54
ASTm1–142 5% TFE	4.43	1.33
ASTm1–142 5% Glycerol	3.65	1.85
ASTm1–142 10% TFE	6.04	1.38
ASTm1–142 10% Glycerol	4.22	1.50
ASTm1–142 15% TFE	6.73	1.13
ASTm1–142 15% Glycerol	5.08	1.86
Tm143–284 (5OHW269)	1.57	2.59
Tm143–284 (5OHW269) 5% TFE	2.91	2.98
Tm143–284 (5OHW269) 5% Glycerol	1.98	3.18
Tm143–284 (5OHW269) 10% TFE	4.16	2.29
Tm143–284 (5OHW269) 10% Glycerol	2.48	2.55
Tm143–284 (5OHW269) 15% TFE	5.50	1.72
Tm143–284 (5OHW269) 15% Glycerol	3.10	3.03