TABLE 2.

Effect of pH on membrane dipole potential

pH	F (di-8 ANEPPS)	Ψd (mV)
2	N/A	N/A
3	1.5801 ± 0.0102*	221.9 ± 9.4*
4	1.6027 ± 0.0330*	222.7 ± 9.8*
6.5	1.5635 ± 0.0215*	215.5 ± 6.2*
9	1.5084 ± 0.0381	205.3 ± 11

Values are shown as mean ± SD. Experiments were repeated at least three times.

^*Indicates values are statistically different from pH 9, as determined by a Student's t-test at 95% confidence. The ANOVA test supports the hypothesis that pH has an effect on SOPC dipole potential (F = 11.2 and P = 8.9 × 10⁻⁶).