FIGURE 2.

Characterization of hK421C's motility on microtubules using single-molecule wide-field fluorescence microscopy assays. (A) Histogram of the velocities of individual motors (N = 657). The solid line is a Gaussian fit to the distribution with its center position at 886 ± 7 nm/s (mean ± SE). (B) Distribution of the probability of being bound after a given run length (N = 204). A single exponential fit (solid line) indicates that the average run length is 1550 ± 30 nm (not corrected for photobleaching). The error bars are calculated from the square root of the absolute number of already detached motors and normalized.