FIGURE 10.

Behavior of P2X₇-dependent single channels in a patch of an intact oocyte. Example of a patch-clamp record in the cell-attached mode. As pipette solution, a U-tube solution containing 0.1 mM ATP⁴⁻ was used. (A, B) Example current traces recorded 3 and 10 min after formation of the gigaseal, respectively. V_h = −60 mV. (C, E, F) Amplitude, open and closed time histogram, respectively, of the record obtained between 10 and 10.5 min after gigaseal formation. (D) Current-voltage relationship of the same patch. The slope conductance was determined by a linear fit of the data between −80 and −20 mV.