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Change in fluorescence values (Δfi) attributed to each state (Ci) in the kinetic model
| Cysteine | Dye | Δf1 | Δf2 | Δf3 | Δf4 | Δf5 |
|---|---|---|---|---|---|---|
| C255 | TMR5M | 1.8 | 1.3 | 1.2 | 0 | −0.9 |
| C511 | TMR5M | 1.8 | −2.5 | 1 | −1.1 | −16.5 |
| TMR6M | 1.5 | 0.5 | −1.2 | −4 | −3.6 |
These values, given in percentage of the fluorescence level recorded at +75 mV, were used to simulate the steady-state fluorescence (ΔF) as a function of membrane potential (Vm).
