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. 2007 Jan 19;92(7):2516–2522. doi: 10.1529/biophysj.106.098723

FIGURE 1.

FIGURE 1

Representative nonlinear regression fits of the ITC-integrated heat data for netropsin binding to the AATT containing OHP (◊), LSHP (Δ), and DLSHP (○) DNA constructs at 298 K (25°C). The fits shown as solid lines are for the “two-fractional-sites” model described by Eqs. 1 and 2. This model is represented by two fractional sites that are constrained to have a combined stoichiometry of 1.0 mol of ligand/mol of DNA. The fit lines shown above were obtained with a user-defined fitting routine coded in Mathematica 5.0 by our laboratory.