TABLE 2.
ITC-derived thermodynamic parameters, ΔGi, ΔHi, and −TΔSi for the OHP DNA construct binding netropsin in MES buffer (0.01 M MES, 0.001M EDTA, 0.2 M NaCl, pH 6.2) at a range of temperatures from 275 to 328 K
| Temp (K) | ΔG1* (kcal/mol) | ΔH1* (kcal/mol) | −TΔS1 (kcal/mol) | ΔG2* (kcal/mol) | ΔH2* (kcal/mol) | −TΔS2 (kcal/mol) |
|---|---|---|---|---|---|---|
| 275 | −9.7 | −3.1 | −6.6 | −7.7 | −10.3 | 2.6 |
| 278 | −9.2 | −3.3 | −5.9 | −7.4 | −9.2 | 1.8 |
| 288 | −9.7 | −6.3 | −3.4 | −8.0 | −12.2 | 4.2 |
| 298 | −11.2 | −7.1 | −4.1 | −8.3 | −12.0 | 3.7 |
| 310 | −10.9 | −12.4 | 1.5 | −8.6 | −21.7 | 13.1 |
| 318 | −11.2 | −11.9 | 0.7 | −8.8 | −26.6 | 17.8 |
| 323 | −11.4 | −14.3 | 2.9 | −8.4 | −36.1 | 27.7 |
| 328 | −8.9 | −18.4 | 9.5 | na | na | na |
The parameters are for the “two-independent-sites” binding model.
*
The uncertainties (not shown here) for fit parameters ΔG1, ΔG2, ΔH1, and ΔH2 were taken directly from the fitting program and were typically <2% of the listed parameter value.