TABLE 3.
ITC-derived thermodynamic parameters, ΔGi, ΔHi, and −TΔSi, for the LSHP DNA construct binding netropsin in MES buffer (0.01 M MES, 0.001 M EDTA, 0.2 M NaCl, pH 6.2) at a range of temperatures from 275 to 333 K
| Temp (K) | ΔG1 (kcal/mol) | ΔH1 (kcal/mol) | −TΔS1(kcal/mol) | ΔG2 (kcal/mol) | ΔH2 (kcal/mol) | −TΔS2 (kcal/mol) |
|---|---|---|---|---|---|---|
| 275 | −9.8 (±0.2) | −3.6 (±0.5) | −6.2 | −8.3 (±1.3) | −14.8 (±2.0) | 6.5 |
| 278 | −9.5 (±0.1) | −3.4 (±0.5) | −6.1 | −8.3 (±0.4) | −13.1 (±0.5) | 4.8 |
| 288 | −10.0 (±0.2) | −5.1 (±0.6) | −4.9 | −8.3 (±0.4) | −14.2 (±0.7) | 5.9 |
| 298 | −10.9 (±0.5) | −7.1 (±0.5) | −3.8 | −8.5 (±0.2) | −15.9 (±2.3) | 7.4 |
| 310 | −10.9 (±0.1) | −9.5 (±0.8) | −1.4 | −8.6 (±0.2) | −23.4 (±1.4) | 14.8 |
| 318 | −11.2 (±0.1) | −12.2 (±0.3) | 1.0 | −8.7 (±0.2) | −32.8 (±3.4) | 24.1 |
| 323 | −11.1 (±0.2) | −−12.0 (±1.3) | 0.9 | −8.5 (±0.2) | −39.8 (±3.1) | 31.3 |
| 328 | −11.2 (±0.2) | −13.5 (±2.0) | 2.3 | −8.3 (±0.2) | −44.1 (±4.2) | 35.8 |
| 333 | −11.4 (±0.4) | −13.2 (±1.9) | 1.8 | −8.3 (±0.2) | −46.8 (±2.8) | 38.5 |
The parameters are for the “two-independent-sites” binding model. The uncertainties listed for ΔGi and ΔHi are ±1 standard deviation from the mean values determined by averaging triplicates for each experiment.