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. 2007 Mar;170(3):909–920. doi: 10.2353/ajpath.2007.060665

Figure 3.

Figure 3

Double labeling for TUNEL (green) and 4-HNE (red) in villous tissues fixed immediately after delivery (A), in control untreated explants after a 6-hour exposure to H/R (B), and in GTN-treated explants after a 6-hour exposure to H/R (1 μmol/L and 1 nmol/L GTN; C and D, respectively). E: Western blot and densitometric analysis performed using explants from three to six placentas confirmed the above results. The asterisk indicates a significant difference (P < 0.05) between the levels of 4-HNE in untreated villi after a 6-hour H/R insult compared with the levels of 4-HNE in control villi processed immediately after delivery. Scale bars = 30 μm.