Figure 5.

Lack of sustained neutrophil infiltration after reperfusion of ischemic kidneys in the absence of cathepsin G. Wild-type 129/SvJ and cathepsin G^−/− mice were subjected to 60 minutes of warm ischemia. Kidneys were retrieved 9 or 24 hours after reperfusion, and frozen sections were prepared and stained to detect neutrophils. Renal sections are from wild-type 129/SvJ mice at 9 hours after reperfusion (a), cathepsin G^−/− mice at 9 hours after reperfusion (b), wild-type 129/SvJ mice at 24 hours after reperfusion (c), and cathepsin G^−/− mice at 24 hours after reperfusion (d). Magnification, ×200. e: The numbers of neutrophils were counted in 10 microscopic (magnification, ×200) fields/slide for four slides/kidney and four kidneys per group. The mean number of neutrophils per field ± SD is shown for each group. **P < 0.001.