Figure 5.

TcsL-82-induced plasma EPO increases and does not modify renal and hepatic functions. Mice were intraperitoneally injected with 0.5 ml of HBSS containing or not TcsL-82 (15 ng/mouse). At the indicated times, eight mice were euthanized, and sera were collected. The amount of EPO present in plasma of control or TcsL-82-intoxicated mice was estimated by ELISA using a R&D kit as indicated in the Materials and Methods section. Horizontal bars indicate the mean values. The Wilcoxon signed-rank test was performed between the values of the control versus intoxicated mice at each time point using arbitrarily the median of control batch as the hypothetical value for comparison. The probability P of a significant difference in TcsL-82-intoxicated mice is indicated: *0.05 > P > 0.01, **0.01 > P > 0.001 for each time point. Renal and hepatic functions were analyzed by measuring on the one hand BUN and CRE and, on the other hand, alanine aminotransferase and aspartate aminotransferase from serum at 0, 6, and 16 hours after intraperitoneal injection of 15 ng of TcsL-82. n represents the number of sera studied for each group.