Figure 7.

TcsL-82 modifies adherens junctions and actin cytoskeleton in lung vessels and glucosylates small GTPases in lung. Lung (A) and heart (B) vessel cryosections of control and TcsL-82-treated mice for 6 and 18 hours were analyzed with an anti-mouse VE-cadherin detected with a relevant secondary antibody coupled to fluorescein isothiocyanate, and with tetramethyl-rhodamine isothiocyanate phalloidin. C: Lungs and liver of mice intoxicated for 0, 6, and 16 hours with intraperitoneally injected TcsL-82 (15 ng/mouse) were homogenized and glucosylation with [¹⁴C]UDP-glucose in the presence of TcsL-82 was performed as previously reported.28 Autoradiograph of the gel shows the amount of in vivo glucosylated GTPases after TcsL-82 intoxication. Quantification of the radiolabeled bands was performed using Denylab 2.5.2. software (bottom; DynaLab Inc., Hong Kong). The experiments shown are representative of three different ones.