Figure 8.

TcsL-82 increases the level of certain chemokines, primarily in lung and plasma and, to a lesser extent, in liver. Mice were injected intraperitoneally with 0.5 ml of HBSS containing or not TcsL-82 (15 ng/mouse). After the indicated times, mice were euthanized and sacrificed, blood, lung, and liver were collected. Plasma was sampled after centrifugation. After weighing, organs were homogenized and centrifuged, and the extracted supernatants were collected. KC, MIP-1α, MIP-2, and JE/MCP-1 were measured by ELISA using R&D kits in each sample from lungs (A), plasma (B), and liver (C). Chemokine measurements represent individual values of six samples for each time point. Horizontal bars represent the median value at each time point. The Wilcoxon signed-rank test was performed between the values of the control versus intoxicated mice at each time point using arbitrarily the median of the control population as the hypothetical value for comparison. The probability P of a significant difference in TcsL-82-intoxicated mice is indicated: *0.05 > P > 0.01; NS, nonsignificant.