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T-cell priming and cytokine production during EAE. A: Lymph node cells were recovered 13 days after EAE induction and stimulated with ConA or gpMBP. The mitogenic response was determined by [3H]thymidine incorporation assay (N = 4). B–D: The levels of IL-4, IL-10, and IFNγ were measured by cytokine bead array in the culture supernatant of lymph node cells 72 hours after stimulation with ConA or gpMBP, respectively (N = 3). Filled bars, wild type; open bars, transgenic.
