Figure 10.
Macrophage TGF-β and VEGF-A expression in myocardial cryolesions of control mice and mice intravenously injected with CL2MDP liposomes 7 days after injury. A and B: Immunofluorescent double staining using an anti-TGF-β antibody (A1 and B1, red) and an anti-F4/80 antibody for macrophages (A2 and B2, green) after PBS (A) or CL2MDP liposome (B) injection. Arrows in the merged micrographs (A3 and B3) indicate a selection of co-localization of TGF-β and macrophages. C: Immunofluorescent double staining using an anti-VEGF-A antibody (C1, red) and an anti-F4/80 antibody (C2, green) for macrophages after PBS (A) injection. Arrows in the merged micrograph (C3) indicate a selection of co-localization of VEGF-A and macrophages. A high level of TGF-β and VEGF-A is present in the cryolesion (A1 and C1). The merged micrographs show co-localization of TGF-β (A3) and VEGF-A (C3) with macrophages in the cryolesion. TGF-β (B) and VEGF-A (data not shown) expression was markedly reduced in the cryolesions of macrophage-depleted mice. Magnification, ×400.