RT-PCR detection of transcript expression of Ang-sNPF and Ang-sNPFR genes in body regions of An. gambiae life stages. Embryos (E), and heads (H), thoraces (T) and abdomens (A) of 4th instar larvae (L4), pupae, males and non-blood fed females are shown. The size of RT-PCR products for Ang-sNPF is 445 bp and for Ang-sNPFR is 620 bp. The presence (+) or absence (−) of PCR products in each of the three cohorts tested is shown below each representative gel photo panel. Control PCR reactions included template with forward (FP) or reverse (RP) primers only, and primers with no template (NT).