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. Author manuscript; available in PMC: 2007 May 2.
Published in final edited form as: J Biol Chem. 2005 May 12;280(27):25769–25779. doi: 10.1074/jbc.M502356200

FIG. 2. SNAP-29-EGFP inhibits repetitive (0.1 Hz) synaptic transmission but has no effect on basal synaptic transmission in cultured hippocampal neurons.

FIG. 2

A, a representative pair of neurons demonstrates a synaptic connection between the presynaptic neuron transfected with SNAP-29-EGFP (indicated by the white image in the bottom panel) and the untransfected postsynaptic neuron during patch clamp recording. B, individual traces were recorded from different pairs of synapses. An overlay of four sweeps of PSCs includes the first two (marked as a) and the last two responses (marked as b) during 15 min of repetitive stimulation (0.1 Hz) from the neurons expressing SNAP-29-EGFP (top panel) or EGFP (middle panel) and untransfected control neurons (bottom panel). C, normalized amplitude of PSC plotted against time (in minutes). The tendency curves of three groups of the synapses were smoothed by the adjacent five data points (Origin Pro version 7.0 software). D, histograms of the normalized PSC among groups of synapses during different time periods (in seconds) of recording. Each column represents mean ±S.E. (**, p < 0.01, Wilcoxon rank-sum test). E, representative PSCs from neurons presynaptically expressing SNAP-29-EGFP (left) or EGFP (right). Steady synaptic responses within the first minute of recordings were selected for assessment of basal synaptic transmission. All recordings were performed 12–24 h post-transfection on hippocampal cultures at DIV 8–10. F, histograms of basal synaptic transmission representing mean PSC amplitudes in neurons presynaptically expressing SNAP-29-EGFP (105 ± 29 pA) or EGFP control (89 ± 22 pA) (p > 0.05).