Table 2.
Primer names and sequences, PCR annealing temperatures and product lengths for five exons of EFNB2 and three exons (five amplicons) of SHH
Key: f, forward primer; r, reverse primer.
| Primer | Primer sequence 5′ to 3′ |
PCR annealing temperature (°C) |
PCR product length (bp) |
|---|---|---|---|
| EFNB2_1f | GGAGCTGGGAGTGGCTTC | 65 | 257 |
| EFNB2_1r | GAACCGAGGTTCCAGAAACA | ||
| EFNB2_2f | CATTCATCCCATATTGATCTGA | 62 | 630 |
| EFNB2_2r | TAGCACAGGGTCCCAAATTC | ||
| EFNB2_3f | CAGCATCCCCATACTTTCAGA | 64 | 340 |
| EFNB2_3r | CGGCAACATTAGCACCATACT | ||
| EFNB2_4f | TGCAGCTCCTAAAGCTAGATATTGT | 64 | 324 |
| EFNB2_4r | CACTGACAGCAAAGATTCCTGA | ||
| EFNB2_5f | TGCACTGGGACAGCAGTAACT | 65 | 499 |
| EFNB2_5r | GAGGCATCGGGACATTAGGT | ||
| SHH_1f | AGGAAGAGAAGAGCGAGGC | 68 | 582 |
| SHH_1r | CGGGTGAAATCACCTTCCTA | ||
| SHH_2f | CGGCTCTTCGGTGTGTCT | 62 | 512 |
| SHH_2r | CCCCCAGGTTTCTTTTTCTC | ||
| SHH_3af | AGGAATGTGTCGGAATCCTG | 65 | 374 |
| SHH_3ar | GGAAAGTGAGGAAGTCGCTG | ||
| SHH_3bf | CAGCGACTTCCTCACTTTCC | 59 | 367 |
| SHH_3br | GTTGATGAGAATGGTGCCCT | ||
| SHH_3cf | GTGACCCTAAGCGAGGAGG | 55.5 60 |
459 322 |
|
SHH_3cr UPIIIA_HaeIII_f |
GTGTTTTTGCTTTGCGTTGC GTATGAAGGTCCCCAAGCAGC |
||
| UPIIIA_HaeIII_r | TCAGGGTGTGACAACCACCG |