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. Author manuscript; available in PMC: 2008 Mar 23.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Jan 23;354(4):955–961. doi: 10.1016/j.bbrc.2007.01.072

Fig 1. Effect of SpA on NF-κB activation in HUCL and primary HCECs: dose and time-dependent course studies.

Fig 1

HUCL (A, B & D) and Primary (C) cells were incubated with various concentrations of SpA for 1 h (A), or 100 μg/ml for different time points (B & C) with unstimulated cells as control. A, B, and C, Western blotting of total cell lysate for phospho-IκB-α (IκB-α) and total IκB-α. D. NF-κB p65 DNA binding activity in HUCL nuclear extracts measured by ELISA using a Colorimetric Transfactor p65 kit.. Results shown are representative of three independent experiments.