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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Clin Immunol. 2006 Dec 20;122(3):259–270. doi: 10.1016/j.clim.2006.11.002

Figure 2. JWH-015 induces apoptosis in naïve and activated splenocytes in vitro.

Figure 2

A) Splenocytes from C57BL/6 were cultured in medium alone or in the presence of 2 μg/ml Con A, 5 μg/ml anti-CD3 mAbs, or 5 μg/ml LPS for 48hrs. In addition, these cultures received 20 μM JWH-015 or the vehicle. The cells were harvested and analyzed for apoptosis using the TUNEL assay followed by flow cytometry. The percentage of apoptotic cells after exposure to JWH-015, obtained by subtracting the empty histogram (vehicle control) from the filled histogram (cells treated with JWH-015) has been depicted in each histogram. B) Splenocytes from C57BL/6 mice were incubated with various concentrations of JWH-015 (0, 5, 10, and 20 μM) or the vehicle for 24hrs. The induction of apoptosis was then analyzed using the caspase-3/7 Apo-One fluorimetric assay. The data represent the mean ± SEM of duplicate cultures. Asterisk indicates statistically significant difference when compared to vehicle control.