Figure 5.

CBP is required for the transcriptional activation of Wg endogenous targets genes in Kc cells. (A–E) Cells were treated for 4 days with control dsRNA (10 μg/well) or different doses (7.5 and 10 μg/well) of dsRNA corresponding to CBP. Cells were then incubated for 5 h with control or WCM before transcript levels of nkd (A), notum (B), α-tubulin (C), arm (D) and TCF (E) were measured by quantitative RT–PCR as described in Materials and methods. Results for nkd and notum were normalized to the average of α-tubulin, arm and TCF expression, while the later three were normalized to total RNA. Wg activation of nkd and notum were reduced in CBP-depleted cells. (F) Western blot showing that induction of Arm protein and TCF protein levels were not affected by CBP depletion.