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. 2007 Apr 5;26(9):2284–2294. doi: 10.1038/sj.emboj.7601667

Figure 7.

Figure 7

CBP is recruited to the WRE of the nkd gene in a Wg- and Arm-dependent manner. (A, B) ChIP using antibodies against TCF (A) and Arm (B) demonstrate enhanced binding to a cluster of TCF binding sites in the nkd intron (compared with the nkd ORF) in Kc cells stimulated with WCM for 4 h. (C) Kc cells were transfected with an Arm* expression plasmid and an hsp70 luciferase reporter containing a 420 bp fragment of the nkd intron (nkd-luc) or the same sequence with five predicted five TCF destroyed by site-directed mutagenesis (nkdmut-luc). Reporter gene activity was assayed as described in Figure 3 and Materials and methods. Arm* activated nkd-luc 30-fold but did not activate nkdmut-luc. (D) CBP is recruited to the nkd WRE in a Wg and arm-dependent manner. Cells were transfected with control or arm dsRNA and cultured for 4 days before treatment with control or Wg-CM for 4 h before lysis and ChIP analysis with anti-CBP antisera. Precipitated DNA were purified and detected by Q-PCR using primers specifically against the nkd WRE or ORF as described in Materials and methods. Values are the mean of duplicate precipitations (±standard deviations) and the data is expressed as percentage of input DNA.