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. 2007 Apr 12;26(9):2339–2349. doi: 10.1038/sj.emboj.7601681

Figure 2.

Figure 2

Accumulation of proteasomal subparticles in β5Δpro β7HC cells. (A) Superose-6 chromatography followed by SDS–PAGE and anti-HA immunoblot analysis of whole-cell extracts from WT (MHY2498) and β5Δpro (MHY2506) cells. Alleles encoding β2-HA2 and Ump1-HA2 proteins replaced the corresponding endogenous loci. Peak positions of mature proteasomes, proteasome precursor complexes, and free subunits are indicated at the top of each panel. (B) Superose-6 column fractions resolved by nondenaturing PAGE with subsequent anti-HA immunoblotting. Proteasomes and proteasome subparticles are indicated at right (see Figure 3 legend). Ferritin (437 kDa) and thyroglobulin (669 kDa) were used as approximate size markers.