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Isolation of the reconstituted central domain RNPs. The reconstitution mixture was loaded on 5–20% linear sucrose gradient in Rec20 buffer and centrifuged for 17 h at 33,000 rpm at 4°C in a Spinco SW41 rotor. The fractions (0.5 ml) indicated by the larger symbols (fractions 5–8) were pooled for subsequent analysis.
