Figure 3.

Phylogenetic relationships between Arabidopsis MYB transcription factors and anthocyanin-related MYBs of other species.

(a) MdMYB10 clusters next to PAP1 (AtMYB75), within the anthocyanin MYB regulator subgroup 10. Subgroup numbers are those described by Stracke et al. (2001) and are shown as a suffix after most MYB descriptors. Arabidopsis genes are identified by Arabidopsis unique identifiers. (b) Phylogeny of MdMYB10 and MYB genes from other species involved in the regulation of anthocyanin biosynthesis. Full-length sequences were aligned using Clustal W (opening = 15, extension = 0.3) in Vector NTI 9.0. Conserved motifs were extracted and re-aligned as above. Phylogenetic and molecular evolutionary analyses were conducted using MEGA version 3.1 (Kumar et al., 2004) using a minimum evolution phylogeny test and 1000 bootstrap replicates. Accession numbers for genes from other species are given in Figure 2.